SELECTIVE HEAT INACTIVATION OF PNEUMOCOCCAL TRANSFORMING DEOXYRIBONUCLEATE
نویسندگان
چکیده
منابع مشابه
Bacterial inactivation of transforming deoxyribonucleate.
Competent and noncompetent bacteria are able to bind deoxyribonucleate (DNA). The consequence of this binding is different for the two types of bacteria. Competent bacteria are able to utilize the DNA for transformation. DNA exposed to noncompetent bacteria loses its biological activity with a coincident reduction in the double-stranded molecular weight. The reduction in molecular weight does n...
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The different genetic markers of pneumococcal transforming DNA are inactivated in solution at characteristic temperatures, analogous to melting points. These temperatures are spaced sufficiently to permit inactivation of the lower melting markers while the others are preserved intact.' Such partially inactivated DNA has been considered to be a mixture of native and denatured molecules.1 If this...
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The characteristics of the intermediates in the reaction, between DNA and pneumococcus, that results in genetic transformation are described in so far as they have been characterized. Transformation with DNA isolated from bacteria carrying in addition to genetic markers (32)P as a radioactive label and (2)H and (15)N as density labels has permitted the characterization of the product of recombi...
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Reilly, Bernard E. (Western Reserve University, Cleveland, Ohio), and John Spizizen. Bacteriophage deoxyribonucleate infection of competent Bacillus subtilis. J. Bacteriol. 89:782-790. 1964.-Phenol extracts of the Bacillus subtilis bacteriophages phi1, phi25, and phi29 contained infectious deoxyribonucleic acid. The infectivity was destroyed by catalytic amounts of deoxyribonuclease but not by ...
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1. The transforming substance of Pneumococcus Type III is inactivated by treatment with ascorbic acid. This effect of ascorbic acid is catalyzed by traces of cupric ion and is prevented by the presence of sulfhydryl compounds. 2. Under certain conditions, the activity of transforming substance treated with ascorbic acid can be restored by the use of glutathione and other sulfhydryl compounds. 3...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1961
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.47.5.653